|Year : 2018 | Volume
| Issue : 2 | Page : 68-73
Critical role of Ghrelin in downregulation of the inflammatory response after renal injury
Teba Jasim Mohammed1, Najah R Hadi1, Israa Al-Yasiri2, Nasser Ghaly Yousif3, Abdullah Jasim1, Fadhil Alamran4, Ying Liu5
1 Department of Pharmacology and Therapeutics, University of Kufa, Kufa, Iraq
2 Department of Microbiology, University of Kufa, Kufa, Iraq
3 Department of Medicine, Faculty of Medicine, University of Al Muthanna, Al Muthanna, Iraq
4 Department of Cardiothoracic surgery, Faculty of Medicine, University of Kufa, Kufa, Iraq
5 Department of Medicine, University Beijing, China
|Date of Web Publication||26-Sep-2018|
Najah R Hadi
Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Kufa, Kufa
Source of Support: None, Conflict of Interest: None
BACKGROUND: The impairment of blood flow to the kidney causes renal injury occurs by ischemia/reperfusion (I/R) in surgical procedures, in which the kidneys remain without blood supply for sometime. This is observed during kidney transplantation, vascular surgery of aorta and renal arteries, and in partial nephrectomy. Ghrelin is an orexigenic growth hormone secretagogue of a 28 amino acid peptide derived mainly from ghrelinergic cells in the gastrointestinal tract. It plays an important physiological role in regulating of energy homeostasis and appetite stimulant. In this study, we aimed to assess the ability of ghrelin to exert potential protective effects on the kidney I/R and subsequent kidney dysfunctions in the rat through the modulation of pro-inflammatory cytokines.
MATERIALS AND METHODS: A total of 28 rats were used in this experiment and the I/R was induced in a rat model by unilateral renal artery clamping for 30 min and reperfusion for 3 h, and ghrelin (40 μg/kg/rat) or vehicle was injected through intraperitoneal before ischemia and at reperfusion time immediately. Ghrelin administration could exert a protective role in the kidney against injury by significantly reducing serum and tissue levels of pro-inflammatory markers (tumor necrosis factor-alpha, high mobility group box protein 1, and interleukin 1 beta), decreasing the serum levels of (urea and creatinine), and reduction of the severity score of renal damage.
CONCLUSIONS: We conclude that ghrelin has renoprotective effects through modulation of inflammation.
Keywords: Ghrelin, high mobility group box protein 1, ischemia, reperfusion, tumor necrosis factor-alpha
|How to cite this article:|
Mohammed TJ, Hadi NR, Al-Yasiri I, Yousif NG, Jasim A, Alamran F, Liu Y. Critical role of Ghrelin in downregulation of the inflammatory response after renal injury. Vasc Invest Ther 2018;1:68-73
|How to cite this URL:|
Mohammed TJ, Hadi NR, Al-Yasiri I, Yousif NG, Jasim A, Alamran F, Liu Y. Critical role of Ghrelin in downregulation of the inflammatory response after renal injury. Vasc Invest Ther [serial online] 2018 [cited 2019 Feb 17];1:68-73. Available from: http://www.vitonline.org/text.asp?2018/1/2/68/242262
| Introduction|| |
I/R injury (IRI) is a life-threatening process and a major cause of acute renal failure associated with high mortality and morbidity rates. There are mediators rapidly initiating after direct tissue subjected to hypoxia, namely, tumor necrosis factor-alpha (TNF-α) and interleukin 1 beta that result from ischemia and decrease blood and oxygen supply, ATP production followed by generation of free radicals and later macrophages and T-cells stimulation in response to the time of injury., However, it was found that T-cells is another mediators for renal IRI due to its interaction with renal tubular epithelial cells.
As the I/R was a mechanism of multiple organ failures, it could be the leading cause of release of reactive oxygen species (ROS) which initiate progression of cellular damage in the early phase of reperfusion. Despite the well-documented role of ROS in I/R, the employing of antioxidant agents failed to produce a beneficial therapeutic effect, so many strategies are recognized to prevent excessive production of ROS. The elevated levels of ROS have been related with elevated apoptosis in proximal tubuli but not shown in distal tubuli.
Ghrelin is a common 28-amino acid peptide with an n-octanoyl modification at serine-3, and this conferred its physiological activity. It created by the ghrelinergic cells in the gastrointestinal tract, and consider as an endogenous ligand for growth hormone, which function as a regulation of appetite and assumes a prominent part in controlling the rate of delivery and utilization of energy. Ghrelin applies wide physiological activities in the body involving anti-inflammatory functions, reproductive system, gastric secretion and gastrointestinal motility, glucose homeostasis, cardiovascular functions, in addition to bone formation. It is released in a variety of tissues such as the kidney, testis, pituitary, duodenum, ileum, jejunum, colon, heart, pancreas, lung, and hypothalamus. Ghrelin has the ability to stimulate propagation of the cells of the intestine and inhibits apoptosis during inflammatory condition in addition to oxidative stress. Recent studies show the ability of ghrelin to produce beneficial protective effects in renal I/R in a rat model  injury through vagus nerve, so we have investigated the protective role in renal IRI through modulation of antioxidant and anti-inflammatory effects.
| Materials and Methods|| |
Twenty-eight adult male Sprague-Dawley rats in a weight ranging between 200 and 300 g were obtained from Animal Resource Center, the National Center for Drug Control and Researches. The animals were healthy, and they were kept in the animal house of College of Pharmacy/University of Kufa in a temperature (24°C ± 2°C) until the start of experiments with ambient humidity and were permitted free access to water and standard chow diet. After 1 week of acclimatization in a quarantine room, the rats were randomly divided into four groups, each had seven rats as follow:
Sham group: Rats were underwent anesthetic and surgical procedures same as control group without renal IRI
- Control group: Rats underwent renal ischemia for 30 min by clamping of the left kidney after nephrectomy to the right kidney and reperfusion for 3 h
- Vehicle pretreated group: rats pretreated with normal saline water (vehicle for ghrelin) and subjected to 30 min ischemia and reperfusion for 3 h through intraperitoneal injection (IP)
- Ghrelin-treated group: Rats pretreated with ghrelin 30 min before ischemia IP (40 μg/kg/rat) and at reperfusion time.
Experimental model of renal ischemia/reperfusion injury
- Rats were anesthetized using an intraperitoneal injection of ketamine in a dose of 100 mg/kg and xylazine in a dose of 10 mg/kg. Under sedation (5–10 min), rats were placed on its back, fixed their limbs, and tail with stickers to ensure their stability during surgery. Hair in the chest area was shaved and the skin disinfected. The reflexes were checked through pinching the tail and the hind feet to be sure that the rats were sufficiently anesthetized
- By making midline laparotomy incision to expose the abdomen and in order to expose renal pedicles, the intestines were retracted
- Right renal nephrectomy using the unilateral model of ischemia, the microvascular clamp was positioned around the left renal pedicles. The total time of clamp was 30 min and through this procedure, the abdomen covered using warm and moist gauze. The blood flow to the kidney was returned after the clamp was removed and this could be confirmed visually. Then, the abdominal cavity incision will be closed in two layers
- The animals returned to their cages and allowed to get better with food and water. In postsurgery after 3 h, the animals are euthanized and both blood as well as tissue samples were collected for analysis.
Preparation of samples
At the end of the experiment, blood was gathered from the heart. The sample of blood was placed in a plane tube at 37°C without anticoagulant and left for 30 min, then it was centrifuged at 3000 rpm for 10 min, and then serum obtained used for the determination of serum TNF-α, IL-1 beta (IL-1 β), high mobility box protein 1 (HMGB1), urea, and creatinine by enzyme-linked immunosorbent assay (ELISA). The kit of ELISA was commercially available according to the manufacturer's instructions.
Tissue sampling for measurements of tumor necrosis factor-alpha, interleukin 1 beta, and high mobility group box protein 1
The kidney was washed with normal saline to remove any blood, stored in deep freeze (−80°C), and then homogenization was done by weighing renal tissue (that had been previously stored in the deep freezer) with high-intensity liquid processor in 1:10 (w/v) phosphate-buffered saline and protease inhibitor cocktail. The homogenate was centrifuged at 3000g at 4°C for 20 min. The supernatant was collected for determination of TNF-α, IL-1 β, and HMG-B1 by ELISA with a commercially available ELISA kit (literature of kit) according to the manufacturer's instructions.
Tissue sampling for histopathology
The tissue sample was fixed in 10% formalin and processed by routine histological methods and embedded in paraffin block. The tissue slide sections were cut about 5-μm thick horizontal and stained with hematoxylin and eosin then sent to histopathologist for subsequent histological examination. After fixation, an investigator who was blinded to the experimental treatment groups performed an evaluation of scores. Tissue sections were examined by light microscopy and graded for degeneration/necrosis. Using quantitative measurements for the assessing scoring system of tissue damage., The damage of tubular identified as swelling of tubular epithelial, brush border losing, vacuolar degeneration, and formation of the cast. The degree of damage in kidney was described through the subsequent criteria 0, represents normal; 1, represents <25% of the damage of tubules; 2, represents 25%–50% of damage of tubules; 3, represents 51%–75% of damage of tubules; and 4, represents 75%–100% of damage of tubules.
Data were summarized, analyzed, and presented using two software programs, the Statistical Package for the Social Sciences SPSS version 20 (SPSS Inc., IBM, Chicago, USA) and Microsoft Office Excel 2010. Variables included in the present study were initially described in terms of a number of cases, mean, standard deviation, and range, then they were subjected to two tests of normality distribution Kolmogorov–Smirnov and Shapiro–Wilk tests. Mann–Whitney U-test was used to study the difference in mean rank values between any two groups. Significant level was considered at P ≤ 0.05, whereas highly significant level was regarded at P ≤ 0.01.
| Results|| |
Effects of ghrelin on renal function induced by reperfusion after renal ischemia
Blood urea nitrogen (BUN) and serum creatinine (SCr) levels, two important indexes of kidney function, were significantly increased in the saline-treated I/R group after 3 h of reperfusion, while in the ghrelin-treated group, these levels of BUN and SCr were markedly decreased [P< 0.05, [Figure 1]a and [Figure 1]b.
|Figure 1: Blood urea nitrogen and serum creatinine levels (* significant vs.sham; ** significant vs. control [Control]; P ≤ 0.05)|
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Effects of renal ischemia/reperfusion on inflammatory parameters
In the control group, there are significant (P< 0.05) elevation of serum and tissue levels of TNF-α, IL-1 β, and HMGB1 as compared with sham group. The following figures show these changes [Figure 2], [Figure 3], [Figure 4].
|Figure 2: Mean serum level of tumor necrosis factor-alpha in four experimental groups (* significant vs. sham; # significant vs. control; ¥ significant vs. vehicle; P ≤ 0.05; P ≤ 0.01)|
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|Figure 3: Mean tissue level of tumor necrosis factor-alpha in four experimental groups (* significant vs. sham; # significant vs. control; ¥ significant vs. vehicle; P ≤ 0.05; P ≤ 0.01)|
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|Figure 4: Mean serum level of interleukin-1 beta in four experimental groups (* significant vs. sham; # significant vs. control; ¥ significant vs. vehicle; P ≤ 0.05; P ≤ 0.01)|
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Effect on renal histopathology
The following photomicrograph sections will show the intersection of different scores after 3 h of renal I/R in [Figure 5] and [Figure 6].
|Figure 5: Photomicrographs of kidney sections stained with (H and E, ×40) illustrating. Severity scores: A shows normal renal tubule in the sham group (score 0) marked (1), B shows moderate score of control group with cytoplasmic hyper-eosinophilia (1) and nuclear shading representing “Karyolysis” (2), C shows mild score of ghrelin group withcytoplasmic eosinophilia and vacuolization, D shows moderate score of vehicle group with increased eosinophilia (1), cytoplasmic and nuclear degeneration (2) and cast formation (3)|
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|Figure 6: Mean histological score in four experimental groups (*significant vs. sham; # significant vs. control; ¥ significant vs. vehicle; P < 0.05)|
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| Discussion|| |
The process of abrupt impairment of blood flow and oxygenation to any organ caused by a disturbance in pro-inflammatory cytokines and ROS due to hypoxia and reperfusion induction are known as IRI. The acute renal injury was the suspected results of renal I/R leading to high morbidity and mortality rates.
Influence of renal ischemia/reperfusion on the inflammatory cytokines parameters (tumor necrosis factor-alpha, interleukin-1 beta, and high mobility group box protein 1)
In facts, renal I/R is an inflammatory mechanism cause elevation of inflammatory cytokines (TNF-α, IL-1 β) as shown by  cellular factors that induced due to hypoxia cause endothelial damage and as well as a tubular injury which is the distinctive character of renal damage. In this study, we show significant (P< 0.05) increase in the serum and tissue levels of cytokines as reported through many studies due to the extreme production of cytokines in renal I/R in a rat model. The importance of pro-inflammatory cytokines released during the pathogenesis of cellular injury (TNF-α, IL-1 β) also reported by Chiao et al. which resulted from the leukocyte infiltration and the explanation of this elevated level of cytokine through I/R is that these indicators are produced from macrophages and monocytes, these results agree with the studies , and I/R lead to infiltration of macrophage to the renal parenchyma., The other potent pro-inflammatory cytokine that contributes to the pathogenesis of diverse inflammatory and infectious disorders, namely, HMGB1 is significantly higher in I/R group (P< 0.05) than in a sham group and this is consistent that reported. HMGB1 stimulate proinflammatory responses through TLR4 as proved by a study.
Influence of ghrelin on inflammatory cytokines (interleukin-1, tumor necrosis factor-α, and high mobility group box protein 1)
This study shows that using ghrelin (40 μg/kg/rat) 30 min before induction of ischemia and immediately at the reperfusion time caused significant lowering (P< 0.05) in serum levels of TNF-α, IL-1 β, and HMGB1 as compared with that in I/R group. These findings are consistent with other study. The HMGB1 rapidly translocates from its normal site of residence in the nucleus to the cytoplasm and out of cells when stimulation by injury or by damage signaling molecules. The present study shows that the mean of serum and tissue levels of HMGB1 significantly decrease (P< 0.05) in the ghrelin group. This study also agrees with other study in that ghrelin has an anti-inflammatory effect and inhibit HMGB1 as shown by  which proved the inhibitory role of ghrelin through growth hormone receptor (GHR) downregulated receptor. The administration of recombinant HMGB1 to GHR-treated mice abolished the therapeutic effect of the neuropeptide on sepsis.
Effects of ischemia/reperfusion and ghrelin on renal function parameters (urea and creatinine)
In this study, I/R showed a significant increase (P< 0.05) in serum levels of urea and creatinine when we compared with sham group. These findings agree with most of the evidence that proved the ability of ghrelin to cause a direct effect in reduction of serum levels of urea and creatinine. Rajan et al. showed that administration of human ghrelin as a single dose directly after ischemia (i.e., at the beginning of reperfusion) could protect the kidneys from IRI by attenuating tissue injury indicators (creatinine, BUN).
Effects of ischemia/reperfusion and ghrelin on renal parenchyma
In I/R group, most intersections of renal tissue of rats showed tubular epithelial swelling, loss of brush border, vascular degeneration, necrotic tubules, cast formation, and desquamation. These changes were in agreement with other study  and  also  reported that unilateral renal pedicle clamping for 30 min followed by 24 h of reperfusion in mice would cause renal tissue dysfunction and worse histology after bilateral pedicle clamping with proximal tubular injury, loss of brush-border, proteinaceous casts, interstitial widening, and necrosis and these findings agree with the present study. The severity of histological renal damage is dependent on ischemia time (30 min of unilateral IRI), caused prominent renal damage and severe loss of structure.,,,, The mean severity scores of the sections of the kidney in ghrelin potentiated animals was significantly decreased compared to I/R group (P< 0.05) and that suggest a significant degree of kidney protection from IRI.
Waseem T et al. reported that ghrelin protected kidney parenchyma from severe damage in I/R group by lowering the modulating mediators of inflammation, ROS, and inhibited neutrophil infiltration. At the end of this study and from findings that obtained, it can be concluded that ghrelin protects the kidney with significant amelioration of renal IRI as evidenced by preservation of renal histology with normal renal function parameters and significant reduction of markers of renal injury.
| Conclusions|| |
We conclude that ghrelin has renoprotective effects through modulation of inflammation.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
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